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OBJECTIVE@#Utilize high-resolution chromosome analysis and microarray detection to determine the genetic etiology of infertility of a 32-year old female patient.@*METHODS@#The peripheral blood of the patient was cultured for high-resolution chromosome G and C banding karyotype analysis, and then 750K SNP-Array chip detection was performed.@*RESULTS@#Karyotype analysis results showed that the patient's karyotype was 45,XX,-13 [7]/46,XX,r(13) (p13q34) [185]/46,XX,dic r(13;13)(p13q34;p13q34) [14]/ 47,XX,+der(13;13;13;13) (p13q34;p13q34;p13q34; p13q34), dic r(13;13) [1]/ 46,XX [3]. The microarray results showed that the patient had a 3.3 Mb deletion in the 13q34 segment of chromosome 13, which may be related to infertility.@*CONCLUSION@#Infertility of the patient reported in this article may be related to the deletion of chromosome segment (13q34-qter).
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Adult , Female , Humans , Chimera , Chromosome Banding , Chromosome Deletion , Chromosome Disorders/genetics , Dacarbazine , Infertility/genetics , Ring ChromosomesABSTRACT
Objective To observe the intervention effect of electro-acupuncture(EA) on mammalian target of rapamycin(mTOR) expression in hypoxic-ischemic encephalopathy(HIE) newborn rats. Methods Seven-day-old SD rats were randomly assigned into 3 groups, namely sham-surgery group, HIE model group, and EA group. All of the groups were subdivided into 4 subgroups in terms of 4 time periods of 1, 3, 7 and 21 day(s). Hematoxylin-eosin staining was used for the observation of histological changes in the affected cerebral region. Expression levels of cerebral mTOR were detected with Western blotting method. Results The results of HE staining showed that EA group at experimental day 21 had clear organizational structure, ordered arrangement of neurons, relieved cellular swelling, well cell integrity with clear nucleolus and proliferative glial cells. Compared with the sham-surgery group, mTOR level of the model group was dramatically increased on the 1st, 3rd, 7th and 21st day(P<0.05), and the mTOR expression level of EA group was significantly higher than that in the model group at the above time points (P < 0.05). Conclusion EA can promote mTOR expression in brain tissue and can protect the brain of newborn rats from HIE.
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ConclusionBushen-Yitalpowder adjuvant to progesterone therapy can improve the clinical outcome in patients with early threatened abortion.
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Objective To study the brain functional activation under speech stimulation in presbycusis pa‐tients using blood oxygenation level dependent functional magnetic resonance imaging (BOLD - fMRI) .Methods Eighteen normal hearing young volunteers ,10 normal hearing elderly people and 10 presbycusis patients received fM ‐RI under auditory task .Experimental tasks included monosyllabic verbal stimulating at tone of 1 kHz and sound in‐tensity to 90 dB ± 3 dB(after ambient noise detector Smart Sensor AR844 measurement and calibration) randomly transmitted to left and right ear .Following block design ,each stimulus was repeated twice .The lateralization of brain activation analysis observed changes in hemispheric dominance cases (or ear advantage change) .We observed brain activation analysis and changes of hemispheric lateralization advantage (or ear advantages) .SPM5 software was used to deal with the original pictures in the off - line work station to get fictional maps ,volume and intensity of the acti‐vated brain regions of interest ,and calculate the laterality index (LI) .Results To young people ,regardless of left or right ear stimulation ,the contralateral (region of intrest ,ROI) activation volume and intensity significantly increased compared to ipsilateral ,appearing contralateral hemispheric dominance ,and laterality index were - 0 .58 (left ear stimulation) and 0 .37 (right ear stimulation) .Compared to young people ,elderly people with normal hearing and presbycusis contralateral hemisphere conduction advantage was significantly reduced under monaural stimulation ,and the laterality indexes were - 0 .24 ,0 .28 (the aged group) ,- 0 .18 ,0 .25 (presbycusis group) .Young people received the left and the right ear auditory task respectively ,and the ratio of the total volume of bilateral ROI activation was 1 :2 .06 ,appearing to the right ear advantage (left brain dominance) .While the aged and presbycusis group manifes‐ted as right ear advantage decreased (left ear advantage enhanced) ,whose the total volume ratio was 1 :1 .51 for the old normal subjects and 1 :1 .61 for the hearing impaired seniors .Conclusion Auditory verbal tasks activated brain regions in the superior temporal gyrus and middle temporal gyrus were most obvious .Aging may cause these brain regions to be activated weaker ,appearing to the trend of contralateral hemisphere .The aging and deafness lead to the cortical reorganization .This may be one of the reasons for the declined speech recognition .
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Objective To analyze the factors affecting the effects of hearing aids in presbycusis patients . Methods One hundred and twenty cases with presbycusis were investigated by using general data and the Satisfac-tion with Amplification in Daily Life questionnaire(SADL) .The individual scores and total scores of all the subjects were collected together with the personal information ,including age ,gender ,degree of education ,pure-tone aver-age ,hearing aid type ,monaural/binaural status ,total hearing aid experience ,daily use time ,the time of perceived hearing difficulty .A single factor analysis and multiple stepwise regression analysis were used to filter out main fac-tors influencing the effect of hearing aids .Results The degree of education ,hearing aid type ,monaural/binaural status ,daily use time ,the time of perceived hearing difficulty were factors influencing the effect of hearing aids . The patients with higher level of education ,the longer daily use ,binaural status obtained more scores than those of the low degree of education ,the shorter daily use ,and monaural status( P< 0 .01) .The older people felt better than the youngers(P<0 .01) .The patients with long -time perceived hearing difficulty got more scores than those with short -time perceived hearing difficulty (P<0 .01) .In general ,the degree of education ,and the daily use time were the main factors influencing the effects of hearing aids .Conclusion There are many factors that influence the effects of hearing aids ,in which the degree of education ,daily use time are the main factors ,and the combination of the two factors obtain the effects of hearing aid maximization ,and the binaural status ,the short time of perceived hearing difficulty and the older obtain better satisfaction .The gender doesn't significantly influence the effects of the hearing aids .
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In this paper,we made an analysis of the organization and content settings of medical laboratory students' summer clinical practices,and summarizes the students' achievements and experience of clinical practices in many years.Standardized organization and reasonable content settings are the guarantees for the students to have a comprehensive understanding of the basic major contents.Through the summer clinical practices,students can have a good understanding of their major in short period of time.
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BACKGROUND: Studies concerning trunk muscles mainly focus on muscle strength changes in patients with low back pain,which is rare regarding angle ranges that easily lead,to spinal injury.OBJECTIVE: To explore the mechanics characteristic of flexor-extensor and angle ranges easily lead to spinal injury. METHODS: Fourteen healthy, male, post-graduates of Soochow University were selected. Flexor-extensor strength and spinal angle of participants were measured using CON-TREX isokinetic testing system. All participants took isokinetic centrifugal exercise maximally at the speeds of 30, 60, 90, 120, 180 (°)/s, respectively, with a 5 minutes interval. Main outcome measures:①Lumbar range of motion; ②Peak torque, flexor/extensor, the angle at peak torque at isokinetic centripetal exercise; ③Average power and total work at isokinetic centripetal exercise.RESULTS AND CONCLUSION: ①During isokinetic centripetal exercise, the peak torque of extensor were decreased as the geniovelocity increased (P ＜ 0.05), however, no inerratic changes could be found in flexor peak torque. Though the flexor/extensor was increased with geniovelocity increased, the difference had no significance (P ＞ 0.05). ②Dudng slow isokinetic centripetal exercise underwent different geniovelocity, the angle at peak torque of flexor and extensor distributed discretely, -48.56° at 30 (°)/s; -46.18° at 90 (°)/s; when underwent fast isokinetic exercise, the angles at peak torque of flexor and extensor were very close, which were -48.71° and -51.61° at 120 (°)/s, and -54.86° and -53.11° at 180 (°)/s. ③During isokinetic centripetal exercise, when underwent different geniovelocity, total work of flexor and extensor was reduced with geniovelocity expedited, extensor total work was more than that of flexor (P < 0.05); the average power of flexor and extensor was increased linearly with geniovelocity expedited, and the average power of flexor was less than that of extensor all along (P ＜ 0.01). During isokinetic centripetal exercise, trunk muscle strength descended with the geniovelocity expedited, and the stability of trunk is preferable in slow exercise. During fast isokinetic centripetal exercise, angles at flexor and extensor peak torque are more discrete, which easily result in muscle damages and spine unstable when suffered a suddenly outside attack. Total work of flexor and extensor are descended with the geniovelocity expediting during isokinetic exercise, but the intramuscular eruption increased.